FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002047733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537640
Sequences flagged as poor quality0
Sequence length45-265
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG529349.845621605535303No Hit
ACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG285155.303734841157652No Hit
ACATGGGTCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCT197783.678669741834685No Hit
GCATGGGTCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCT190843.5495870842943233No Hit
GCATGGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCAT190743.547727103638122No Hit
GCATGGGTATGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCA152872.843352429134737No Hit
GCATGGGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTG112752.097128189866825No Hit
ACATGGGTATGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCA83521.5534558440592217No Hit
GCATGGGGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCT72091.3408600550554273No Hit
ACATGGGATCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGC67441.2543709545420727No Hit
GCATGGGCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCC64861.2063834536120823No Hit
ACATGGGTACTGGGAGCGCCTACTACAACCCGTCCCTCAAGAGTCGAGTC63321.1777397515065842No Hit
GCATGGGTACTGGGAGCGCCTACTACAACCCGTCCCTCAAGAGTCGAGTC60351.1224983260174095No Hit
ACATGGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCAT60291.1213823376236887No Hit
ACATGGGATACTGGGAGCGCCTACTACAACCCGTCCCTCAAGAGTCGAGT53971.0038315601517744No Hit
GCATGGGAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAGC52140.9697939141432929No Hit
GCATGGGATCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGC50830.9454281675470575No Hit
GCATGGGAAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAG46580.8663789896585075No Hit
ACATGGGAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAGC45090.83866527788111No Hit
GCATGGGATACTGGGAGCGCCTACTACAACCCGTCCCTCAAGAGTCGAGT40580.7547801502864371No Hit
ACATGGGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTG39740.7391563127743471No Hit
GCATGGGGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTCTGTG39050.7263224462465591No Hit
GCATGGGGAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAG35710.6641990923294397No Hit
GCATGGGGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTCTGTGACCGCC35420.6588051484264563No Hit
ACATGGGGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTCTGTGACCGCC35320.6569451677702551No Hit
GCATGGGACTGCTTCTCATATGCAGGCAGCTACACCCATTGGGTGTTCGG34870.6485752548173499No Hit
GCATGGGGACACGGCCGTGTATTACTGTGCGAACCAACCCAGTAGTTCGT34670.6448552935049475No Hit
GCATGGGTACAACCCGTCCCTCAAGAGTCGAGTCGCCATATCACTGGACA33340.6201175507774719No Hit
GCATGGGTGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAAG33280.6190015623837513No Hit
GCATGGGAGGACATAGTGGAAAACACCCTGACCCCTCTGCCTGGCATAGA31450.5849639163752698No Hit
GCATGGGTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCT30850.5738040324380627No Hit
ACATGGGGACACGGCCGTGTATTACTGTGCGAACCAACCCAGTAGTTCGT30670.5704560672569006No Hit
GCATGGGACTACAACCCGTCCCTCAAGAGTCGAGTCGCCATATCACTGGA30420.5658061156163976No Hit
GCATGGGGGACACGGCCGTGTATTACTGTGCGAACCAACCCAGTAGTTCG30150.5607841678446545No Hit
ACATGGGGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTCTGTG29580.5501822781043078No Hit
GCATGGGAGGGAATATCTATTATACTGGGAGCGCCTACTACAACCCGTCC29450.5477643032512463No Hit
GCATGGGCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTC29300.5449743322669444No Hit
ACATGGGAAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAG28920.53790640577338No Hit
GCATGGGAAGAGTCGAGTCGCCATATCACTGGACACGTCCAAGAACCAAT28250.5254445353768321No Hit
ACATGGGTACAACCCGTCCCTCAAGAGTCGAGTCGCCATATCACTGGACA28170.5239565508518711No Hit
GCATGGGGGAAGGGGCTGGAGTGGATAGGGAATATCTATTATACTGGGAG28010.5209805818019493No Hit
GCATGGGGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAAGC27480.511122684324083No Hit
ACATGGGTATCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAG27200.5059147384867198No Hit
GCATGGGAAGCCAACAAGGCCACACTGGTGGTGCCTCAGCCATGGCCTGG26550.49382486422141203No Hit
ACATGGGGGACACGGCCGTGTATTACTGTGCGAACCAACCCAGTAGTTCG26370.49047689904025No Hit
ACATGGGACTACAACCCGTCCCTCAAGAGTCGAGTCGCCATATCACTGGA26230.48787292612156835No Hit
GCATGGGGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGG25400.4724350866750986No Hit
GCATGGGCAGGGAAGGGGCTGGAGTGGATAGGGAATATCTATTATACTGG24650.45848523175358974No Hit
GCATGGGAGACTGAGGATGAGGCTGATTATTACTGCTTCTCATATGCAGG22860.42519157800758867No Hit
GCATGGGGCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGC22290.414589688267242No Hit
ACATGGGGGAAGGGGCTGGAGTGGATAGGGAATATCTATTATACTGGGAG22220.41328770180790125No Hit
ACATGGGGAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAG21850.4064057733799568No Hit
GCATGGGTAGGGAATATCTATTATACTGGGAGCGCCTACTACAACCCGTC21850.4064057733799568No Hit
GCATGGGGGGAGCGCCTACTACAACCCGTCCCTCAAGAGTCGAGTCGCCA21330.39673387396771076No Hit
GCATGGGGGAGTGGATAGGGAATATCTATTATACTGGGAGCGCCTACTAC21030.3911539319991072No Hit
ACATGGGGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCT20760.38613198422736406No Hit
GCATGGGTATCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAG20500.38129603452124095No Hit
GCATGGGGGGAATATCTATTATACTGGGAGCGCCTACTACAACCCGTCCC19550.3636262182873298No Hit
ACATGGGACTGCTTCTCATATGCAGGCAGCTACACCCATTGGGTGTTCGG19270.3584182724499665No Hit
GCATGGGAGGGGCTGGAGTGGATAGGGAATATCTATTATACTGGGAGCGC19230.35767428018748604No Hit
ACATGGGCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTC18400.34223644074101633No Hit
ACATGGGCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCC17190.31973067480098205No Hit
GCATGGGGAAGGGGCTGGAGTGGATAGGGAATATCTATTATACTGGGAGC17100.318056692210401No Hit
GCATGGGGAGTCGCCATATCACTGGACACGTCCAAGAACCAATTCTCCCT17040.3169407038166803No Hit
ACATGGGGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGG16940.31508072316047914No Hit
GCATGGGTACTGAGGTCAGGGGCTCCCCAGGTGGACACCAGGACTCTGAC16270.30261885276393125No Hit
ACATGGGAAGAGTCGAGTCGCCATATCACTGGACACGTCCAAGAACCAAT15870.29517893013912655No Hit
ACATGGGAGGGAATATCTATTATACTGGGAGCGCCTACTACAACCCGTCC15330.2851350345956402No Hit
ACATGGGGGAGTGGATAGGGAATATCTATTATACTGGGAGCGCCTACTAC14460.26895320288669No Hit
GCATGGGAGTCTGGGCTTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG14080.26188527639312553No Hit
GCATGGGGCCGTGTATTACTGTGCGAACCAACCCAGTAGTTCGTCCCAGG14000.26039729186816457No Hit
GCATGGGGGGAGGAACCTGCTCAGTCTGGGCCTAAGGAAGCAGCACTGGT13190.24533144855293504No Hit
GCATGGGAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCC13160.24477345435607467No Hit
ACATGGGTGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAAG12930.24049549884681198No Hit
ACATGGGGGGAGCGCCTACTACAACCCGTCCCTCAAGAGTCGAGTCGCCA12760.23733353173126998No Hit
GCATGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCT12650.2352875530094487No Hit
GCATGGGAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCC12580.23398556655010788No Hit
GCATGGGGGGGTCTCAGGAGGCAGCACTCTCGGGACGTCTCCACCATGGC12580.23398556655010788No Hit
GCATGGGACGGCCTCCCTGACCATCTCTGGGCTCCAGACTGAGGATGAGG12560.23361357041886766No Hit
ACATGGGAGACTGAGGATGAGGCTGATTATTACTGCTTCTCATATGCAGG12430.2311955955658061No Hit
GCATGGGACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTCT12390.23045160330332567No Hit
ACATGGGAGGGGCTGGAGTGGATAGGGAATATCTATTATACTGGGAGCGC12380.2302656052377055No Hit
GCATGGGGTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCC12260.2280336284502641No Hit
ACATGGGGTACTGGGGCTGGATTCGCCAGCCCCCAGGGAAGGGGCTGGAG12140.2258016516628227No Hit
ACATGGGTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCT12130.2256156535972026No Hit
ACATGGGGGGAATATCTATTATACTGGGAGCGCCTACTACAACCCGTCCC11790.2192917193661186No Hit
GCATGGGGGATAGGGAATATCTATTATACTGGGAGCGCCTACTACAACCC11540.21464176772561563No Hit
GCATGGGTCAGAAGTAATAGTCAGTACTGGGGCTGGATTCGCCAGCCCCC11500.2138977754631352No Hit
ACATGGGTAGGGAATATCTATTATACTGGGAGCGCCTACTACAACCCGTC11460.21315378320065473No Hit
ACATGGGTACTGAGGTCAGGGGCTCCCCAGGTGGACACCAGGACTCTGAC11310.21036381221635295No Hit
ACATGGGGAAGGGGCTGGAGTGGATAGGGAATATCTATTATACTGGGAGC11150.20738784316643105No Hit
GCATGGGAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCG10790.20069191280410684No Hit
GCATGGGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAAGCT10720.19938992634476604No Hit
ACATGGGTACTGGGGCTGGATTCGCCAGCCCCCAGGGAAGGGGCTGGAGT10690.19883193214790565No Hit
GCATGGGGAGGAACCTGCTCAGTCTGGGCCTAAGGAAGCAGCACTGGTGG10660.19827393795104534No Hit
ACATGGGGGATAGGGAATATCTATTATACTGGGAGCGCCTACTACAACCC10650.19808793988542517No Hit
GCATGGGATATGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACC10630.19771594375418497No Hit
ACATGGGACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTCT10590.1969719514917045No Hit
GCATGGGGCTGGGGTCTCAGGAGGCAGCACTCTCGGGACGTCTCCACCAT10260.1908340153262406No Hit
GCATGGGATGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAA9920.1845100810951566No Hit
GCATGGGATCAGAAGTAATAGTCAGTACTGGGGCTGGATTCGCCAGCCCC9640.17930213525779332No Hit
ACATGGGCAGGGAAGGGGCTGGAGTGGATAGGGAATATCTATTATACTGG9380.17446618555167026No Hit
GCATGGGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTC9310.17316419909232944No Hit
ACATGGGGAGTCGCCATATCACTGGACACGTCCAAGAACCAATTCTCCCT9260.17223420876422885No Hit
ACATGGGAGGACATAGTGGAAAACACCCTGACCCCTCTGCCTGGCATAGA9220.17149021650174837No Hit
GCATGGGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGACTG9190.17093222230488803No Hit
GCATGGGGAAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAA9060.1685142474518265No Hit
ACATGGGATTATACTGGGAGCGCCTACTACAACCCGTCCCTCAAGAGTCG8820.16405029387694367No Hit
ACATGGGTCAGAAGTAATAGTCAGTACTGGGGCTGGATTCGCCAGCCCCC8680.16144632095826203No Hit
GCATGGGAGTGATGTTGGTTATTATAACTATGTCTCCTGGTTCCAACAAC8340.15512238672717804No Hit
ACATGGGATCAGAAGTAATAGTCAGTACTGGGGCTGGATTCGCCAGCCCC8320.15475039059593781No Hit
GCATGGGGGGGCTGGAGTGGATAGGGAATATCTATTATACTGGGAGCGCC8170.15196041961163606No Hit
ACATGGGGCCGTGTATTACTGTGCGAACCAACCCAGTAGTTCGTCCCAGG8150.1515884234803958No Hit
GCATGGGTGAGGATGAGGCTGATTATTACTGCTTCTCATATGCAGGCAGC8050.14972844282419462No Hit
ACATGGGAGTCTGGGCTTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG7830.14563648538055204No Hit
ACATGGGATATGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACC7510.13968454728070828No Hit
GCATGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCTTCTCATATG7510.13968454728070828No Hit
ACATGGGGCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGC7310.13596458596830593No Hit
GCATGGGAAAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAA7230.134476601443345No Hit
GCATGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGA7150.13298861691838404No Hit
GCATGGGAAACAACATTGGAGGTAAAAGTGTGCACTGGTACCAGCAGAAG7090.13187262852466336No Hit
GCATGGGGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT6990.13001264786846217No Hit
GCATGGGGGATGAGGCTGATTATTACTGCTTCTCATATGCAGGCAGCTAC6930.12889665947474147No Hit
GCATGGGGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGC6920.12871066140912135No Hit
GCATGGGTACTGCTTCTCATATGCAGGCAGCTACACCCATTGGGTGTTCG6880.12796666914664087No Hit
GCATGGGAGGGAAGGGGCTGGAGTGGATAGGGAATATCTATTATACTGGG6860.12759467301540062No Hit
GCATGGGGCGGACACGGCCGTGTATTACTGTGCGAACCAACCCAGTAGTT6830.12703667881854028No Hit
GCATGGGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA6770.12592069042481957No Hit
GCATGGGAGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTG6670.12406070976861841No Hit
GCATGGGTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACC6660.12387471170299828No Hit
ACATGGGGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAAGC6650.12368871363737817No Hit
GCATGGGGCTCAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGC6650.12368871363737817No Hit
GCATGGGGGGGGTCTCAGGAGGCAGCACTCTCGGGACGTCTCCACCATGG6610.12294472137489772No Hit
GCATGGGAGAGTCGAGTCGCCATATCACTGGACACGTCCAAGAACCAATT6590.12257272524365748No Hit
GCATGGGATGATTTATGATGTCACTAAGCGGCCCTCAGGGGTCCCTGATC6510.12108474071869653No Hit
GCATGGGAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGA6410.11922476006249534No Hit
GCATGGGGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAGCA6360.11829476973439478No Hit
ACATGGGGAAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAA6290.11699278327505394No Hit
GCATGGGGAGCTCTGTGACCGCCGCGGACACGGCCGTGTATTACTGTGCG6290.11699278327505394No Hit
GCATGGGGAGTCAGTACTGGGGCTGGATTCGCCAGCCCCCAGGGAAGGGG6290.11699278327505394No Hit
ACATGGGGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAGCA6190.11513280261885275No Hit
GCATGGGTGATGTTGGTTATTATAACTATGTCTCCTGGTTCCAACAACAC6080.11308682389703147No Hit
GCATGGGGAGGCTGATTATTACTGCTTCTCATATGCAGGCAGCTACACCC6020.11197083550331076No Hit
ACATGGGAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCG5980.11122684324083029No Hit
GCATGGGATTATACTGGGAGCGCCTACTACAACCCGTCCCTCAAGAGTCG5940.11048285097834981No Hit
GCATGGGAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGT5930.11029685291272971No Hit
ACATGGGAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCC5890.10955286065024925No Hit
GCATGGGAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACT5870.109180864519009No Hit
GCATGGGGGGCTCCAGACTGAGGATGAGGCTGATTATTACTGCTTCTCAT5840.10862287032214865No Hit
ACATGGGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGACTG5750.1069488877315676No Hit
GCATGGGGAGTCTGGGTCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCAT5500.10229893609106466No Hit
ACATGGGAAAGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAA5450.10136894576296407No Hit
GCATGGGGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGT5450.10136894576296407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)