FastQCFastQC Report
Wed 8 May 2024
EGAF00002047735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379346
Total Bases79 Mbp
Sequences flagged as poor quality0
Sequence length74-288
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAT28590575.36786996567777No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGACCTTGGGCTGACCTAG127093.3502396229299904No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG115303.039441565220142No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAT88042.3208363868341833No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAAT73341.933327358137426No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCCCCCG72101.9006395217031415No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGACCTTGGGCTGACCTAG66841.7619798284415809No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG61831.6299104247837068No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAC50061.3196395902421536No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAGAG46511.2260574778698075No Hit
CTTACCTGAGGAGACGGTGACCAGGGTACCCTGGCCCCAGTAGTCGAAAG40981.0802802718362656No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGTAGCCACAGTTCGTTTGAT16830.4436582961201647No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAA16110.424678262061548No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAG13830.3645748208759286No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGAGGA12850.3387408856294781No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAAG6730.1774105961312364No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAACCACAGTTCGTTTGAT6540.1724019760324348No Hit
TCAGATGGCGGGAAGATGAAGACAGGTGGTGCAGCCACAGTTCGTTTGAT6390.16844780227022296No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTACAGCCACAGTTCGTTTGAT6170.162648347418979No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTCAAT5690.1499949913799012No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGTCACAGTTCGTTTGAT5360.14129580910303524No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGTTGACCTAG5100.13444190791520144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)