FastQCFastQC Report
Wed 8 May 2024
EGAF00002047736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304812
Total Bases276.9 Mbp
Sequences flagged as poor quality0
Sequence length73-288
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCCCCCG85455665.49265334776197No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAT32127024.621937873042246No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG266092.039297615288639No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGAGGA241281.8491552806074747No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAT161441.2372663648096431No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGATATCAAAAG53950.41346952664445147No Hit
CTTACCTGAGGAGACGGTGACCAGGGTACCCTGGCCCCAGTAGTCGAAAG50850.38971131473346354No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCCCCGG39740.3045649488202132No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGACCCCAGAAGTCCCCCG30600.23451654337942937No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTACAGCCACAGTTCGTCTGAT24570.18830298924289476No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGTAGCCACAGTTCGTCTGAT22570.17297511059064447No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGACGCCGT14770.11319638384686835No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCAGAAGTCCCCCGG14180.10867465964445452No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGAAGTCCCCCG13840.10606892027357198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)