FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002047737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128048
Sequences flagged as poor quality0
Sequence length68-288
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG6620951.70639135324253No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG3318525.916062726477573No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAT53924.210920904660752No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGC48633.7977945770336126No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG20981.6384480819692615No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAGAG14961.1683118830438586No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGC12410.9691678120704736No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG12320.9621391978008247No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACCTAG11370.8879482693989754No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGACCTTGGGCTGACCTAG9400.7340997126077721No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAAT5710.445926527552168No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGACCTTGGGCTGACCTAG5520.4310883418717981No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA4950.3865737848306885No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGTAGCCTTGGGCTGACCTAG4620.36080219917530926No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACCTAG4290.33503061351993No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAG3580.2795826565038111No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAG3270.25537298513057605No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCAGTCATGG3060.238972885168062No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGC2620.20461077096088964No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGT2450.191334499562664No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGACTGACCTAG2240.17493439960014995No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGTAGCCTTGGGCTGACCTAG2210.17259152817693366No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA1860.14525802823941023No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGG1840.14369611395726603No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGTCTTGGGCTGACCTAG1630.127296013994752No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAACCTTGGGCTGACCTAG1510.1179245283018868No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGC1400.10933399975009371No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAG1390.10855304260902161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)