FastQCFastQC Report
Wed 8 May 2024
EGAF00002047738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072934
Total Bases221 Mbp
Sequences flagged as poor quality0
Sequence length63-288
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAGAG52136148.59208488126949No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAT27961226.060503255559052No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGAGGA462804.313406043614985No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG395483.68596763640634No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTCAAT328393.0606728838866135No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAA325253.031407337263988No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG271982.5349182708349254No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG251262.341802944076709No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG163291.5219016267543017No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGGT31380.29246906147069623No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCACAATAGTCAAGAG28540.26599958618144265No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGC25400.23673403955881722No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGGCACAGTTCGTTTGAT24650.2297438612253876No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGACCCCAATAGTCAAGAG23550.21949159966969078No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGAGCAGCCACAGTTCGTTTGAT17860.16645944671340457No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGTAGCCACAGTTCGTTTGAT17620.16422258964670708No Hit
CTTACCTGAGGAGACGGTGACCGACGTGCTGTAGACGCGCAGGCCGCTCA15260.14222682849084844No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGAGAGTGAAATCTGTCCCAG12440.115943757957153No Hit
CTTACCTGAGGAGACGGTGACCAGAGTTCCCTGGCCCCAATAGTCAAGAG11590.10802155584593275No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAG11590.10802155584593275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)