FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002047739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379346
Sequences flagged as poor quality0
Sequence length51-265
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGCAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT282627.450190591175339No Hit
GCATGGGAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACTGTCAAC212645.605436725311457No Hit
ACATGGGAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACTGTCAAC99592.625307766524492No Hit
GCATGGGAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACC78442.0677692660526272No Hit
ACATGGGCAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT69241.8252466086369699No Hit
GCATGGGTCAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACTGTCA66731.7590801010159591No Hit
GCATGGGATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTC49551.3061953994506335No Hit
GCATGGGACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGGGGAC49431.303032060440864No Hit
ACATGGGATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTC45691.2044413279697164No Hit
GCATGGGGTGGAGATCAAACGTAAGTGCACTTTGCTAATCCTTTTTATTA41411.091615569954606No Hit
GCATGGGACTACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGGG41291.0884522309448368No Hit
GCATGGGGGGACAGATTTCACTCTCACCATCAGCAATCTGCAACCTGAAG38921.0259762855018901No Hit
GCATGGGATCAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACTGTC38501.014904598967697No Hit
GCATGGGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCTGCAA36130.9524286535247504No Hit
GCATGGGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCC35020.9231677676843831No Hit
GCATGGGTCTGCAACCTGAAGATTCAGCAACTTACTACTGTCAACAATAT34600.9120960811501901No Hit
ACATGGGTCAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACTGTCA34590.9118324695660426No Hit
GCATGGGACTCTCACCATCAGCAATCTGCAACCTGAAGATTCAGCAACTT32970.8691273929341551No Hit
ACATGGGACTACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGGG32950.8686001697658602No Hit
ACATGGGACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGGGGAC32790.8643823844195009No Hit
ACATGGGAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACC29220.77027304887886No Hit
GCATGGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCG29120.7676369330373853No Hit
ACATGGGACTCTCACCATCAGCAATCTGCAACCTGAAGATTCAGCAACTT28700.7565652465031923No Hit
ACATGGGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCTGCAA27920.736003542939691No Hit
GCATGGGACGTCTATGTAAATTGGTATCAGCAGAAACCAGGGAAACCCCC27710.7304676996725944No Hit
GCATGGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTCAAACGA27600.7275679722469724No Hit
GCATGGGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCA27280.719132401554254No Hit
ACATGGGATCAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACTGTC27190.7167598972969268No Hit
GCATGGGAAGATTCAGCAACTTACTACTGTCAACAATATTACAAAGACCC26760.7054245991785864No Hit
GCATGGGCAGCAACTTACTACTGTCAACAATATTACAAAGACCCTACTTT25560.6737912090808919No Hit
GCATGGGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCTGCAACC24770.652965893933243No Hit
ACATGGGGTGGAGATCAAACGTAAGTGCACTTTGCTAATCCTTTTTATTA24240.638994479973428No Hit
GCATGGGAACCTGAAGATTCAGCAACTTACTACTGTCAACAATATTACAA23740.6258139007660553No Hit
ACATGGGTCTGCAACCTGAAGATTCAGCAACTTACTACTGTCAACAATAT23010.6065702551232911No Hit
ACATGGGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCTGCAACC21750.573355195520712No Hit
GCATGGGCAACCTGAAGATTCAGCAACTTACTACTGTCAACAATATTACA20580.5425126401754599No Hit
GCATGGGCATCAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACTGT20370.5369767969083634No Hit
ACATGGGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC19470.5132517543350925No Hit
ACATGGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTCAAACGA18330.48320003374228276No Hit
GCATGGGAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCA18250.48109114106910317No Hit
GCATGGGGCAACTTACTACTGTCAACAATATTACAAAGACCCTACTTTCG17700.46659250394099316No Hit
GCATGGGCACTCTCACCATCAGCAATCTGCAACCTGAAGATTCAGCAACT17440.45973860275315936No Hit
ACATGGGAACCTGAAGATTCAGCAACTTACTACTGTCAACAATATTACAA16980.4476124698823765No Hit
ACATGGGGGGACAGATTTCACTCTCACCATCAGCAATCTGCAACCTGAAG16970.447348858298229No Hit
GCATGGGAATCTGCAACCTGAAGATTCAGCAACTTACTACTGTCAACAAT15990.42151492305177857No Hit
GCATGGGACCATCAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACT15960.4207240882993363No Hit
GCATGGGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC15810.4167699145371244No Hit
GCATGGGGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCC15210.40095321948827717No Hit
ACATGGGACGTCTATGTAAATTGGTATCAGCAGAAACCAGGGAAACCCCC15060.39699904572606537No Hit
GCATGGGAAGTCAGGCCATTCACGTCTATGTAAATTGGTATCAGCAGAAA14980.3948901530528857No Hit
ACATGGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCG14560.38381846651869267No Hit
GCATGGGTCTGGGACAGATTTCACTCTCACCATCAGCAATCTGCAACCTG14380.3790734580040385No Hit
GCATGGGGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCTGCA13790.3635203745393388No Hit
ACATGGGAATCTGCAACCTGAAGATTCAGCAACTTACTACTGTCAACAAT13630.35930258919297947No Hit
GCATGGGAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCA13420.353766745925883No Hit
ACATGGGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCA13300.3506034069161135No Hit
GCATGGGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTT13200.347967291074639No Hit
GCATGGGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCT12750.3361047697880036No Hit
ACATGGGAAGATTCAGCAACTTACTACTGTCAACAATATTACAAAGACCC12620.3326778191940867No Hit
GCATGGGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA12340.325296694837958No Hit
GCATGGGAGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCC12070.3181791820659767No Hit
ACATGGGAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCA11900.31369778513547No Hit
ACATGGGTTACTACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGG11750.3097436113732582No Hit
ACATGGGTATTACAAAGACCCTACTTTCGGCGGGGGGACCAAGGTGGAGA11490.3028897101854244No Hit
ACATGGGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCC11460.30209887543298203No Hit
GCATGGGGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGC11140.2936633047402635No Hit
ACATGGGACCATCAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACT11100.2926088584036737No Hit
GCATGGGAAACCCCCTAAGCTCCTGATCGATACTGTGTCCAGTTTGCAAA10950.2886546846414619No Hit
GCATGGGTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAG10870.2865457919682823No Hit
GCATGGGGACTACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGG10660.28100994870118573No Hit
GCATGGGTACTACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGG10390.2738924359292045No Hit
GCATGGGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAAT10130.2670385347413707No Hit
ACATGGGCAACCTGAAGATTCAGCAACTTACTACTGTCAACAATATTACA9870.2601846335535369No Hit
ACATGGGTCTGGGACAGATTTCACTCTCACCATCAGCAATCTGCAACCTG9760.2572849061279149No Hit
GCATGGGTTACTACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGG9530.25122183969252343No Hit
GCATGGGTATTACAAAGACCCTACTTTCGGCGGGGGGACCAAGGTGGAGA9510.25069461652422853No Hit
ACATGGGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA9140.24094098791077276No Hit
ACATGGGCACTCTCACCATCAGCAATCTGCAACCTGAAGATTCAGCAACT9020.23777764890100334No Hit
ACATGGGTACTACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGG9010.23751403731685586No Hit
GCATGGGGCTCCTGATCGATACTGTGTCCAGTTTGCAAACTGGGGTCCCA8920.23514153305952876No Hit
ACATGGGCATCAGCAATCTGCAACCTGAAGATTCAGCAACTTACTACTGT8770.23118735929731696No Hit
ACATGGGGCAACTTACTACTGTCAACAATATTACAAAGACCCTACTTTCG8690.22907846662413733No Hit
GCATGGGAATATTACAAAGACCCTACTTTCGGCGGGGGGACCAAGGTGGA8630.22749679711925264No Hit
GCATGGGAACCCCCTAAGCTCCTGATCGATACTGTGTCCAGTTTGCAAAC8420.22196095385215606No Hit
GCATGGGTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGGGGACCA7780.20508981246671903No Hit
GCATGGGTGCCGGACAAGTCAGGCCATTCACGTCTATGTAAATTGGTATC7720.20350814296183434No Hit
ACATGGGGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCTGCA7490.19744507652644286No Hit
ACATGGGAATATTACAAAGACCCTACTTTCGGCGGGGGGACCAAGGTGGA7340.1934909027642311No Hit
GCATGGGGTCAACAATATTACAAAGACCCTACTTTCGGCGGGGGGACCAA7290.1921728448434938No Hit
ACATGGGATTACAAAGACCCTACTTTCGGCGGGGGGACCAAGGTGGAGAT7250.19111839850690399No Hit
GCATGGGTACTGTGTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGT7210.1900639521703142No Hit
GCATGGGTCCTGATCGATACTGTGTCCAGTTTGCAAACTGGGGTCCCATC7130.18795505949713454No Hit
GCATGGGATTACAAAGACCCTACTTTCGGCGGGGGGACCAAGGTGGAGAT7090.18690061316054474No Hit
GCATGGGGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATT6990.1842644973190702No Hit
ACATGGGAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCA6910.18215560464589056No Hit
GCATGGGAAGCTCCTGATCGATACTGTGTCCAGTTTGCAAACTGGGGTCC6850.18057393514100584No Hit
GCATGGGAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGC6670.1758289266263517No Hit
ACATGGGGCTCCTGATCGATACTGTGTCCAGTTTGCAAACTGGGGTCCCA6650.17530170345805676No Hit
ACATGGGAAACCCCCTAAGCTCCTGATCGATACTGTGTCCAGTTTGCAAA6650.17530170345805676No Hit
ACATGGGTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGGGGACCA6610.17424725712146694No Hit
GCATGGGACTGTGTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTG6610.17424725712146694No Hit
ACATGGGTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAG6560.1729291992007297No Hit
ACATGGGCAGCAACTTACTACTGTCAACAATATTACAAAGACCCTACTTT6560.1729291992007297No Hit
ACATGGGGACTACTGTCAACAATATTACAAAGACCCTACTTTCGGCGGGG6550.17266558761658224No Hit
ACATGGGGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGC6530.17213836444828734No Hit
ACATGGGTACTGTGTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGT6520.17187475286413986No Hit
GCATGGGAGCAATCTGTAACCTGAAGATTCAGCAACTTACTACTGTCAAC6370.16792057910192804No Hit
ACATGGGAAGTCAGGCCATTCACGTCTATGTAAATTGGTATCAGCAGAAA6190.1631755705872739No Hit
ACATGGGAACCCCCTAAGCTCCTGATCGATACTGTGTCCAGTTTGCAAAC6050.15948500840920954No Hit
GCATGGGTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGG6020.1586941736567672No Hit
GCATGGGAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGT5960.15711250415188244No Hit
GCATGGGTCAGCAGAAACCAGGGAAACCCCCTAAGCTCCTGATCGATACT5910.1557944462311452No Hit
ACATGGGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAAT5860.1544763883104079No Hit
ACATGGGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTT5800.1528947188055232No Hit
GCATGGGCGATACTGTGTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTC5780.1523674956372283No Hit
ACATGGGGTCAACAATATTACAAAGACCCTACTTTCGGCGGGGGGACCAA5750.15157666088478594No Hit
GCATGGGTCGATACTGTGTCCAGTTTGCAAACTGGGGTCCCATCAAGGTT5710.1505222145481961No Hit
GCATGGGGAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTC5680.14973137979575374No Hit
GCATGGGTAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT5660.14920415662745884No Hit
GCATGGGAGCTCCTGATCGATACTGTGTCCAGTTTGCAAACTGGGGTCCC5540.1460408176176894No Hit
ACATGGGTCCTGATCGATACTGTGTCCAGTTTGCAAACTGGGGTCCCATC5420.14287747860791994No Hit
ACATGGGGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCC5420.14287747860791994No Hit
GCATGGGGGGGCCAGAGTCACCATCACTTGCCGGACAAGTCAGGCCATTC5120.13496913108349634No Hit
GCATGGGTCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCT5040.1328602384103167No Hit
GCATGGGAGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCC4880.12864245306395744No Hit
ACATGGGAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGT4860.12811522989566254No Hit
GCATGGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTC4820.12706078355907272No Hit
GCATGGGACCAGGGAAACCCCCTAAGCTCCTGATCGATACTGTGTCCAGT4770.12574272563833544No Hit
GCATGGGAGGCTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAG4740.12495189088589309No Hit
GCATGGGAGCAGAAACCAGGGAAACCCCCTAAGCTCCTGATCGATACTGT4730.12468827930174563No Hit
ACATGGGGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATC4720.12442466771759818No Hit
GCATGGGAGACAAGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTC4640.12231577504441854No Hit
ACATGGGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAATCT4600.12126132870782873No Hit
ACATGGGACTGTGTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTG4550.11994327078709147No Hit
GCATGGGTCAGGCCATTCACGTCTATGTAAATTGGTATCAGCAGAAACCA4540.11967965920294402No Hit
GCATGGGTGTGTCCAGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGC4530.11941604761879657No Hit
GCATGGGGCAGAAACCAGGGAAACCCCCTAAGCTCCTGATCGATACTGTG4440.11704354336146948No Hit
GCATGGGTTACAAAGACCCTACTTTCGGCGGGGGGACCAAGGTGGAGATC4280.11282575801511022No Hit
ACATGGGTTACAAAGACCCTACTTTCGGCGGGGGGACCAAGGTGGAGATC4280.11282575801511022No Hit
GCATGGGAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACC4240.11177131167852042No Hit
GCATGGGGGGACAGCAGAATACGGTGGATCCGCGAAAAGTACATTCACCA4210.11098047692607803No Hit
GCATGGGAGGGCCACTGGCATCCCAGACAAGTTCAGTGGCAGTGGGTCTG4170.10992603058948823No Hit
GCATGGGGGCCATTCACGTCTATGTAAATTGGTATCAGCAGAAACCAGGG4150.10939880742119332No Hit
GCATGGGAGTCAGACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCG4110.10834436108460352No Hit
GCATGGGGTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATC3970.10465379890653914No Hit
GCATGGGAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACA3850.10149045989676972No Hit
ACATGGGTGCCGGACAAGTCAGGCCATTCACGTCTATGTAAATTGGTATC3850.10149045989676972No Hit
GCATGGGGGGGGCCAGAGTCACCATCACTTGCCGGACAAGTCAGGCCATT3830.10096323672847479No Hit
GCATGGGAGAGTCACCATCACTTGCCGGACAAGTCAGGCCATTCACGTCT3810.10043601356017988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)