FastQCFastQC Report
Mon 9 Jan 2023
EGAF00002048022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002048022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366315184
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG44541901.2159446822166127TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT8755360.23901165942386923TruSeq Adapter, Index 27 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCCCGTATG6333400.1728948260031722TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)