FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002049563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002049563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658634
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1290383.5269447558843003TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC95170.260124407087454TruSeq Adapter, Index 6 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48000.13119650667434896No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)