FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002049573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002049573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739745
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC230033.1095850597165238TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGACCTCGTATGC10790.14586107374838628TruSeq Adapter, Index 1 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8900.1203117290417644No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAACTCGTATGC8720.11787845811732421TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)