FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002050045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3557775
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC761672.140860509728693TruSeq Adapter, Index 11 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46160.12974401135541175No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT41350.11622432559675641TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)