FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002050395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329865
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1736985.216367630519556TruSeq Adapter, Index 12 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT93440.28061197676182065TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGC63190.1897674530348828TruSeq Adapter, Index 12 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61400.18439185973004912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)