FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002050715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552383
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC194453.5202024682149884TruSeq Adapter, Index 9 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT55100.9974963023843963TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC15190.2749903599495278TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC9230.16709420818526277TruSeq Adapter, Index 9 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6290.11387026754987029No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC5690.10300823884876978TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)