FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002050725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310103
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC45451.4656420608636485TruSeq Adapter, Index 11 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT3230.10415894073904476TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)