FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002050727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3456946
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1511704.372934954725935TruSeq Adapter, Index 14 (97% over 44bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN99150.28681385245821023No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTACCTCGTAT78970.22843862762102737TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTAT52930.1531120243127894TruSeq Adapter, Index 14 (97% over 41bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGT40100.115998340731964TruSeq Adapter, Index 14 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)