FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002050817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230312
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT83173.611188301087221TruSeq Adapter, Index 13 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGT9240.40119490083017817TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT5080.22057035673347458TruSeq Adapter, Index 13 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4170.18105873771232067No Hit
GATCGGAAAAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3870.1680329292438084TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)