FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002050818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230312
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG100074.344975511480079Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4210.18279551217478898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)