FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002050827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265021
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT150695.685964508472914TruSeq Adapter, Index 15 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGT13910.5248640673757928TruSeq Adapter, Index 15 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT8790.3316718297795269TruSeq Adapter, Index 15 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7580.2860150705038469No Hit
GATCGGAAAAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT6760.2550741262013199TruSeq Adapter, Index 15 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCC4930.18602299440421702TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAACTCGTAT3040.1147078910727829TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)