FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002050835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336089
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT159044.73207989550387TruSeq Adapter, Index 18 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8550.25439690082091354No Hit
GATCGGAAAAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT7510.2234527163935743TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATATCGTAT6610.1966740952545308TruSeq Adapter, Index 18 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCC4650.13835620921839156TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGT3700.11008988690495672TruSeq Adapter, Index 18 (97% over 35bp)

[OK]Adapter Content

Adapter graph