FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002050843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573302
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT141402.4664138621529315TruSeq Adapter, Index 20 (97% over 44bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGT9010.15715975175387492TruSeq Adapter, Index 20 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATATCGTAT6870.11983213036061273TruSeq Adapter, Index 20 (97% over 43bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT6270.10936644211951119TruSeq Adapter, Index 20 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)