FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002050847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304018
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT161905.325342578399963TruSeq Adapter, Index 21 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGT17770.584504864843529TruSeq Adapter, Index 21 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATATCGTAT9070.2983375984316718TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT7940.2611687465873731TruSeq Adapter, Index 21 (97% over 40bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7920.26051089080251827No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAAACTCGTAT4600.15130683051661414TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)