FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002050963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266600
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT95703.5896474118529635TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGT27891.0461365341335334TruSeq Adapter, Index 7 (96% over 31bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT4410.16541635408852212TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATATCGTAT3740.14028507126781695TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCC3150.11815453863465866TruSeq Adapter, Index 7 (96% over 33bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3040.1140285071267817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)