FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002050967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002050967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253884
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT119874.7214475902380615TruSeq Adapter, Index 25 (97% over 44bp)
GAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGT10880.4285421688645208TruSeq Adapter, Index 25 (97% over 39bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT5410.2130894424225237TruSeq Adapter, Index 25 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATATCGTAT4720.18591167619857887TruSeq Adapter, Index 25 (97% over 43bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3450.13588883111972397No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCC3390.1335255471002505TruSeq Adapter, Index 25 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)