FastQCFastQC Report
Sat 13 May 2023
EGAF00002051965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002051965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50804877
Sequences flagged as poor quality0
Sequence length30-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG1885430.3711120095812849No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC1744040.3432820042060135No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1322910.26039035583139No Hit
GGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCT1265940.24917686544148115No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT1042800.20525588517810994No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT1020930.20095118033648618No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT988900.1946466674843047No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA916630.18042165518873315No Hit
ATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTA864960.17025137173346566No Hit
GGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCC855770.16844249027509703No Hit
CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC846450.16660802072210507No Hit
CGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTA781060.15373720912659625No Hit
CACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG780050.1535384093145231No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT770610.15168031998187892No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG761850.14995607606726416No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG755130.1486333684067378No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC739210.14549981097287176No Hit
AATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAA686380.13510120298096578No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC680250.13389462590372966No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC666670.13122165417308262No Hit
GAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA637110.12540331511874342No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA628970.12380110673233201No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC617360.1215158930509762No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA606250.11932909511817144No Hit
GTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCC605000.11908305574679376No Hit
ATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAAT597580.11762256603829589No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA593980.11691397264872819No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAA585480.11524090492336002No Hit
CCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA573200.11282381413894577No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC564800.1111704295632878No Hit
CTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGG563530.11092045356196807No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC561480.11051694899290869No Hit
TGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTT558310.10989299314709491No Hit
TCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAA554850.10921195616712151No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT549230.10810576315340749No Hit
GAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACA522120.10276966126696852No Hit
TGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGA520150.10238190321767732No Hit
GTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAA511150.10061041974375806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)