FastQCFastQC Report
Sun 14 May 2023
EGAF00002051967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002051967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55657057
Sequences flagged as poor quality0
Sequence length30-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG2807210.5043762914018253No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC2016310.3622739161360975No Hit
GGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCT1585530.28487492610326126No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1493690.2683738739545643No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1410350.2534000315539501No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT1248000.22423032536556864No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA1100710.19776647550731977No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT1085430.19502109139547208No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1062130.19083473996837455No Hit
GGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCC988350.17757855935501585No Hit
ATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCTA984530.1768922133270539No Hit
ATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAAT896160.16101462209904485No Hit
CGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTA833790.1498084959828185No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT821130.14753385181685047No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC820840.14748174701368058No Hit
AATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAA814070.1462653693672664No Hit
CACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG812460.145976097873806No Hit
CAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA766100.13764651623602736No Hit
AGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATA763150.13711648461757509No Hit
TGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTT757290.1360636082500733No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC745480.13394168505891355No Hit
CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC740970.13313136553375432No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT717170.1288551782391225No Hit
GAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA686090.1232709807131915No Hit
AATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAAC668890.1201806268700122No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT666320.11971887051088598No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA665200.11951763816760919No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC664700.11942780230007491No Hit
GTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCC660070.11859592216670746No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA655480.11777122890274275No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA638260.11467728162486204No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC613480.11022501602986302No Hit
AGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAAT580500.10429944220730177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA571690.1027165342213477No Hit
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC569340.10229430564393659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)