FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002063510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002063510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74684625
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9968401.334732550374324No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1471710.19705662310013608TruSeq Adapter, Index 25 (97% over 44bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080710.1447031433845989No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT988580.13236727104139573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)