FastQCFastQC Report
Sun 5 Mar 2023
EGAF00002063522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002063522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65700347
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24597043.7438219314123256No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2866420.4362868890174964No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380000.3622507503651389No Hit
CTCGTGTTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATT1540630.23449343425842178No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1394590.21226524115618445No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1313240.19988326697878778No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1137200.17308888794757812No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1101080.16759120008909542No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA795130.12102371392345918No Hit
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA784510.11940728410460297No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC729890.11109378158992067TruSeq Adapter, Index 1 (100% over 50bp)
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG712730.1084819232385485No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT704220.10718664849669668No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT686520.104492598798603No Hit
GGAGTTTTGGAAGCAAAAGGCCAACATCTTGGGGGTCAGCCAACAGGTCC675610.1028320291824334No Hit
GGCTTTTTGTATACTTCACAGTGGCAACAGGAGCTAGAGCATAGAAGGTT664380.10112275358302142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)