FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002063529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002063529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82532423
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11496851.3930101143401545No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3518910.4263669806471088No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3114150.3773244364823749Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3033330.3675319213638015No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2676360.3242798287892263No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1998410.24213635409686204No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1449940.17568125923069047No Hit
CAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGCTGCT1334260.16166494954352667No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1205060.1460104957781259No Hit
GCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGC1192090.1444389921764444No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1131140.13705401572906686No Hit
CCTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAAC1009900.1223640314061784No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGC995910.12066894001161216No Hit
CGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCC963530.1167456334100357No Hit
GTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCT963160.11670080254398928No Hit
GAGAAACAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTG898550.1088723640162606No Hit
CTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCCTGCCCG883930.10710093898491262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)