FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002063541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002063541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74755144
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37200504.976313068168259No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15774302.1101290367389303No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10863041.4531494983141227No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9707651.2985929102082927No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540581.1424738878170042No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4782120.6397044730460288No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4021320.5379322123973167No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2387930.3194335362393256No Hit
CCCCTCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2095320.28029107936706No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1718440.2298758196492806No Hit
CCCCCTCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1674180.22395515685181477No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1437120.19224362673958598Illumina Single End PCR Primer 1 (100% over 50bp)
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1374070.18380942453940027No Hit
CCCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180060.15785669545362657No Hit
TCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT971450.12995092351102955No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940660.1258321434040713No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA913020.12213473898197562No Hit
CTCCTCTGTCCACTGCTTTCGTGAAGACAAGATGAAGTTCACAATCGTCT823020.1100954337001879No Hit
CTCCTCTGTCCACTGCTTTCGTGAAGACAAGATGAAGTTCACAATTGTCT790060.10568637256587989No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)