FastQCFastQC Report
Thu 23 Feb 2023
EGAF00002063542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002063542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83850457
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47590945.675692381736214No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000650.4771172564986736No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3294510.3929030464318161No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2457900.29312899272570453No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240630.2672173867818037No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1915230.22841020413281707No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1587870.1893692720124352No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1307220.1558989714271921TruSeq Adapter, Index 20 (97% over 44bp)
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA1302510.1553372571362372No Hit
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA1232360.14697117273910623No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT1199800.14308806927551987No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG1118710.1334172811962134No Hit
CTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGC1000740.11934818673677594No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT946970.1129355800648767No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT915940.10923494430089986No Hit
GGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGG898170.10711569526687256No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC886870.10576805800831832No Hit
CTCGTGTTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATT879300.10486526030502134No Hit
GGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCC867960.1035128526490917No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTT854650.10192550292242296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)