FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002066569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9481474
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9327689.837795262635323No Hit
AGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTCT650160.6857161660729123TruSeq Adapter, Index 6 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTC367100.3871760867561309TruSeq Adapter, Index 6 (97% over 34bp)
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT237650.25064668215089764No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA168430.1776411557949745No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG140430.14810988249295415No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC135650.1430684722649664No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC133050.14032628260120736No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC125790.1326692453093264No Hit
GAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTCTT114850.12113095495489414TruSeq Adapter, Index 6 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)