FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002066571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9406897
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5063215.382444391599058No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT678000.7207477662400258No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC447870.47610811514147544No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC407390.43307585912761665No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC357640.3801891314426No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA317280.33728444140506697No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG236980.2519215422471406No Hit
CACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCC172350.18321663349774106No Hit
CCACCGCGCCCGCAGCCCACGGTGTCACCTCGGCCCCGGAGAGCAGGCCGGCCCCGGGCT151280.16081817415455915No Hit
AAGAGCACACGTCTGAACTCCAGTCACATTGGCTCATCTCGTATGCCGTC150710.16021223576701224TruSeq Adapter, Index 12 (96% over 31bp)
GAGAGCAGGCCGGCCCCGGGCTCCACCGCGCCCGCAGCCCACGGTGTCACCTCGGCCCCG149990.15944683990905822No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGAGAGCAGGCCGGCCCCGGGCT115010.12226135781012591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)