FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002066573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9069574
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4050874.4664391072833185No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT209940.23147724468646486No Hit
AAGAGCACACGTCTGAACTCCAGTCACGATGAATCATCTCGTATGCCGTC206330.22749690338267264TruSeq Adapter, Index 9 (96% over 32bp)
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA164590.1814748961748369No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG148330.1635468214934902No Hit
AGAGCACACGTCTGAACTCCAGTCACGATGAATCATCTCGTATGCCGTCT126270.1392237386232253TruSeq Adapter, Index 9 (96% over 31bp)
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC123730.13642316607152663No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC100950.11130622011574082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)