FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002066579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6314550
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1137091.8007458963821652No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA174750.2767418105803264No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC123950.19629268910690392No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT102910.16297281674862024No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA100740.1595363090006414No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG73310.1160969506932402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)