FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002066585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8221474
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4043164.917804276945959No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT380000.4622042227464321No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC277040.3369712049201883No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC214150.26047640605565375No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC187790.22841402891987497No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA163450.198808632126064No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG161600.19655842735743007No Hit
AGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTATGCCGTCT130600.15885229339653692TruSeq Adapter, Index 27 (96% over 32bp)
GAGAGCAGGCCGGCCCCGGGCTCCACCGCGCCCGCAGCCCACGGTGTCACCTCGGCCCCG105890.1287968556489992No Hit
AAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTATGCCGTC100010.12164485346544915TruSeq Adapter, Index 27 (96% over 33bp)
CCACCGCGCCCGCAGCCCACGGTGTCACCTCGGCCCCGGAGAGCAGGCCGGCCCCGGGCT84680.1029985620583365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)