FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002066589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8816092
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2981353.3817138024421705No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA201290.22832112006090682No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG192030.21781759990707902No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT176840.20058774341284094No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC100890.11443846094165079No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC92830.10529608810797347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)