FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002066599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059218
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN544661.341785536031817No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA102610.2527826788312429No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC100350.24721510399293656No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG97060.23911009460442872No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA96220.23704073050523525No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT79490.19582589552963156No Hit
GTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACG67620.16658380998507594No Hit
CACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCC59570.14675240403447168No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG59470.14650605116552007No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC52320.12889182103548025No Hit
CCATGAACTGGAGCTTTATCACCTTCACAATGGAGTACTCACTGCCTATG43370.10684323926431151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)