FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002066615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9211193
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4632345.029033698457952No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT597990.6491992948144719No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC448710.48713559687653923No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC324860.3526796148989604No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC321160.3486627627930497No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA165400.17956414549125177No Hit
AAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTC163640.17765342665168343TruSeq Adapter, Index 6 (97% over 34bp)
GAGAGCAGGCCGGCCCCGGGCTCCACCGCGCCCGCAGCCCACGGTGTCACCTCGGCCCCG146800.15937132139126822No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG134080.14556203523256978No Hit
CACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCC132530.14387929989090445No Hit
CCACCGCGCCCGCAGCCCACGGTGTCACCTCGGCCCCGGAGAGCAGGCCGGCCCCGGGCT126900.13776717087569437No Hit
AGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTCT120790.1311339367224202TruSeq Adapter, Index 6 (96% over 33bp)
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGAGAGCAGGCCGGCCCCGGGCT106910.11606531314673355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)