FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002066619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9489042
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3285043.461930087357607No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT262560.27669811135834366No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA203410.2143630516125864No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC179320.1889758734337987No Hit
AGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTATGCCGTCT167070.17606624567580162TruSeq Adapter, Index 7 (96% over 28bp)
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG163540.17234616518717064No Hit
AAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTATGCCGTC154040.16233461712994843TruSeq Adapter, Index 7 (96% over 29bp)
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC146040.1539038398186034No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC142040.1496884511629309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)