FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002066623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002066623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8928074
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3341063.7421956852060143No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA272510.3052282048737499No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG231910.2597536713965409No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT176160.1973101925454471No Hit
AAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTATGCCGTC138460.1550838400308958TruSeq Adapter, Index 7 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTATGCCGTCT112180.12564860013481072TruSeq Adapter, Index 7 (96% over 28bp)
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC111030.12436052837375676No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC108550.12158277361948389No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC104480.11702411964775383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)