FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002112992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002112992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29020058
Sequences flagged as poor quality0
Sequence length35-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN509188217.546077957528546No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT563340.19412090768391985No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG532950.18364884039859605No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG503980.173666089847236No Hit
TTTATACATAGAATATGCTGAAACAATAGTAACCACCGAAGGTTAGGCA468110.16130567347591104No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT376560.12975852770521684No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT355570.12252559936303366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)