FastQCFastQC Report
Wed 8 May 2024
EGAF00002128760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002128760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425641
Total Bases37.3 Mbp
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123172.893753186370674No Hit
ATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGC27280.640915701259982No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC22570.5302590680878957No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG20420.4797470168522299No Hit
ATGGGCAGAATTCACAATCCGTCTTCACGCTTTGACACCATCGGATTGCT17290.40621086784402816No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT11970.28122290850740417No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGG11220.26360242551821844No Hit
GTGCTGGGCTGTTCGAAAGGCGGGCGAATCAGGAGTCTCGGGCTTTCGGG10590.2488012198073024No Hit
CTCCTAGACTTCATAAGTTCTATGTTTGGTCGTTAATTAGTGGCCAGATG9910.23282531523044067No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA9720.22836145953984693No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT9660.2269518209007121No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC9280.21802410951952464No Hit
GCCCAGCACACTCGACCTATAGAGCCAATCCTTTTCCCTAAGTTACGGAT8810.20698194017963492No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8380.1968795299325018No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA8320.19546989129336695No Hit
ATGGGTAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA8140.19124097537596235No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG8130.19100603560277324No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7820.18372290263390978No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA7810.18348796286072067No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC7240.1700963957889395No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG6210.14589759915045777No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT6180.14519277983089035No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA6030.1416686832330532No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC5960.14002410482072922No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC5760.13532530935694634No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5660.13297591162505493No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT5520.12968675480040692No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGG5510.12945181502721775No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAG5490.1289819354808395No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGTTTAACAACCAACTTAGAA5300.12451807979024576No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG5280.12404820024386749No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA5120.12028916387284119No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT5120.12028916387284119No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT5090.11958434455327377No Hit
GTCTTTGGCCGATGGGAACAAAGTAGCGTAAGGGAATTCGGCAAAATAGA4890.1148855490894909No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4860.11418072976992347No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC4830.11347591045035604No Hit
CTTTGACACCATCGGATTGCTATGTTTTAATTATACAGTTGGATTCTCCT4720.11089157294527548No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4560.1071325365742492No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA4550.10689759680106005No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC4510.10595783770830347No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA4450.10454819906916862No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG4310.10125904224452062No Hit
GCCGAAGCCTGCCAAGGGTGTCTGCGCCCCAGCGCTGAAAGGCGCCCACC4290.10078916269814232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)