FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002128761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002128761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425641
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66581.5642290098933138No Hit
ATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGC21920.5149879828306013No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG17670.4151385792252156No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14820.34818074386630987No Hit
ATGGGCAGAATTCACAATCCGTCTTCACGCTTTGACACCATCGGATTGCT14190.3333795381553939No Hit
CTCCTAGACTTCATAAGTTCTATGTTTGGTCGTTAATTAGTGGCCAGATG8580.20157832539628465No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT8440.19828916857163667No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGG7750.1820783242215858No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA7670.18019880603607263No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT7660.17996386626288352No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC7300.17150603442807438No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG6830.16046386508818464No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6540.15365061166569952No Hit
GCCCAGCACACTCGACCTATAGAGCCAATCCTTTTCCCTAAGTTACGGAT6500.15271085257294292No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA5870.13790964686202692No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA5860.13767470708883778No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA5660.13297591162505493No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC5450.1280421763880829No Hit
ATGGGTAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA5160.12122892296559777No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA4930.11582530818224747No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4860.11418072976992347No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC4760.11183133203803206No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT4500.10572289793511433No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGG4460.10478313884235775No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4360.10243374111046633No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG4280.10055422292495318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)