FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002128958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002128958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888412
Sequences flagged as poor quality0
Sequence length20-100
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC39160.44078648194756487No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG36010.40532995952328427No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32840.36964831632170664No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT21840.24583188880834567No Hit
GTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCCTGAGCGA21450.24144203365105377No Hit
CATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCCTGGGAGGTGGCTG20340.2289478305110692No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA20000.22512077729701985No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG19410.21847971436675775No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17560.19765604246678342No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT17460.19653043858029834No Hit
TGGACTCCATTGATGGTGAAGACGATGTCGGGCAGGCTGCTGATGGCTGA16830.18943913409544222No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC15550.17503140434843295No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15030.16917826413871043No Hit
TGCCTGCACCAACCACAACCGCTTCAACCCTGAGGATTCTTCCACCTACC14850.16715217714303723No Hit
AACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGT14820.16681449597709172No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC13770.15499565516899816No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG13730.15454541361440413No Hit
GTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAA13710.1543202928371071No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA13650.15364493050521605No Hit
GGGTACTGGACTCCATTGATGGTGAAGACGATGTCGGGCAGGCTGCTGAT13330.15004299806846375No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC13290.1495927565138697No Hit
GTCCAGTACCCCGTGCCACCCAGTGCCTACATCCTGCAGAGCGAGGGGAG12180.1370985533738851No Hit
GTCTACCTCAGCGCCGATGACCAGAGTGGCAGCGTGGTGATCTTTGGTGG12110.13631063065334553No Hit
GAGGTAGACAGAGAAGAGGTCCTGAGAAACCAGGCCCTGGTTCCAGATGT11810.13293381899389023No Hit
GGGTGGCACGGGGTACTGGACTCCATTGATGGTGAAGACGATGTCGGGCA11450.12888164500254387No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT11310.12730579956146473No Hit
ACCGTGGACAGCATCACCATGAACGGAGAGGCCATCGCCTGCGCTGAGGG11120.12516715217714303No Hit
GTCCACGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCA10910.12280338401552432No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT10720.12066473663120264No Hit
GGACAGAGGCCAGATCTTCCTGGGAGGTGGCTGAAGAGACTTAGGCTTAA10640.11976425352201456No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC10240.11526183797607417No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10180.11458647564418309No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA10070.11334831136904949No Hit
CCCCTGGGAAGAACAGTCAGGAGAATTAGATACATCTAAAGTGGGCACAG9950.11199758670526738No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC9830.11064686204148524No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA9790.11019662048689122No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT9780.1100840600982427No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA9720.10940869776635165No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG9530.10727005038202996No Hit
CTCCTGCACCATGCCCTGCATAGCTGTGGTGGCCTCATCCACGGACAGAG9520.10715748999338146No Hit
CCAGTACCCCGTGCCACCCAGTGCCTACATCCTGCAGAGCGAGGGGAGCT9200.10355555755662912No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT8900.10017874589717383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)