FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002128961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002128961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770109
Sequences flagged as poor quality0
Sequence length20-100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG24110.3130725650524796No Hit
AACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGT14540.18880444196860444No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14460.18776562798253235No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.17776704336658836No Hit
CATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCCTGGGAGGTGGCTG12840.1667296447645723No Hit
GTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCCTGAGCGA12580.16335349930983797No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12450.1616654265824708No Hit
CTCCTGCACCATGCCCTGCATAGCTGTGGTGGCCTCATCCACGGACAGAG12300.1597176503585856No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT10820.14049959161625172No Hit
TGGACTCCATTGATGGTGAAGACGATGTCGGGCAGGCTGCTGATGGCTGA10780.13998018462321568No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT10370.13465626294459618No Hit
GGGTACTGGACTCCATTGATGGTGAAGACGATGTCGGGCAGGCTGCTGAT9310.1208919776291408No Hit
GTCCAGTACCCCGTGCCACCCAGTGCCTACATCCTGCAGAGCGAGGGGAG9230.11985316364306871No Hit
GTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAA9040.11738598042614747No Hit
TGCCTGCACCAACCACAACCGCTTCAACCCTGAGGATTCTTCCACCTACC8500.1103739860201608No Hit
ACCGTGGACAGCATCACCATGAACGGAGAGGCCATCGCCTGCGCTGAGGG8140.10569932308283633No Hit
CCAGTACCCCGTGCCACCCAGTGCCTACATCCTGCAGAGCGAGGGGAGCT7960.1033619916141741No Hit
GGACAGAGGCCAGATCTTCCTGGGAGGTGGCTGAAGAGACTTAGGCTTAA7760.10076495664899385No Hit
GGGTGGCACGGGGTACTGGACTCCATTGATGGTGAAGACGATGTCGGGCA7760.10076495664899385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)