FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002128994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002128994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366095
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGAGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTT19350.5285513323044565No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGG18820.5140742157090373No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGACGACCCGCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCACCGACAG15400.42065584069708684No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGG13250.36192791488548054No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA11480.3135798085196465No Hit
ACCTATACCCGGCCGTCGGGGCAAGCGCCAGGCCCCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCCGGG11150.30456575479042325No Hit
GTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGTCGCGTGCCGGCCGGGGGACGGACTGGGAACG10470.28599134104535706No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCAGTCCG10310.2816208907524003No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCAGTCCGTCCCCCGGCCGGCACG10290.28107458446578076No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCT8450.23081440609677814No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCCGCCCACCCT7940.21688359578797856No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT7800.21305945178164137No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTC7760.21196683920840217No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGCGCGA7760.21196683920840217No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA7630.20841584834537485No Hit
GTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG7440.2032259386224887No Hit
TCCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGG7050.19257296603340662No Hit
TACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCAGTCCGTCCCCCGGCCG7050.19257296603340662No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCC6730.18383206544749314No Hit
AGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGC6610.18055422772777557No Hit
CCCTGGAAACGACTTAGTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGC6530.1783690025812972No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGG6510.17782269629467762No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCAGTCCGTCCCCC6490.17727639000805803No Hit
TCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGTCGCGTGCCGGCCGGGGGACGGACTGGGAACGG6390.17454485857496005No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAA6340.17317909285841107No Hit
GTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGTCGCGTGCCGGCCG6280.1715401739985523No Hit
GTTCGGGACTGGGACCCCCGAGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGAC6220.1699012551386935No Hit
AAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC6110.16689657056228574No Hit
GAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACGC5980.1633455796992584No Hit
GCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT5920.1617066608393996No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCCGGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTG5370.1466832379573608No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGA5300.1447711659541922No Hit
ATCCTAAGAGACGGGGGAAGCCCGTCCGACAGCGCGTTCGCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC5220.14258594080771386No Hit
GTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCCGCCCACCCTGGAAACGACT5100.1393081030879963No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAA5070.13848864365806687No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGC4930.13466449965172975No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGAC4820.13165981507532198No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4820.13165981507532198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4800.13111350878870237Clontech Universal Primer Mix Long (96% over 26bp)
TTTCCAGGGTGGGCGGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCT4740.1294745899288436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.1292014367855338No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCGATGCCTCTAATCATTGGCTTTACCCG4630.12646990535243582No Hit
GCGCTGAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGCGCCAGGCCCCGATGAGTAGGAGGGCGCGGCGGTC4610.12592359906581627No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC4570.12483098649257707No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTT4390.11991422991300073No Hit
GTTATGAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACAC4390.11991422991300073No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT4390.11991422991300073No Hit
GGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCCCACGCCCGGTCGTACTCATAA4380.11964107676969092No Hit
ATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG4120.11253909504363623No Hit
TCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGA4100.11199278875701661No Hit
GAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCC4020.10980756361053826No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAG3920.10707603217744027No Hit
GGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACGCGACGTGCGGTG3910.1068028790341305No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGGTGCGAGTTCTATCGGGTAAAGCCA3880.10598341960420109No Hit
CCGGACACCACGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGACTAAGTCGTTTCCAGGGTGGGC3790.10352504131441292No Hit
CCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGACGACCCGCTCTCGCCGCGG3780.10325188817110313No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCCGGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGT3740.10215927559786395No Hit
GAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAAT3730.10188612245455415No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAA3710.10133981616793454No Hit
GTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCCCACGCCCGGTCGTACTCATAAC3710.10133981616793454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)