FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002128997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002128997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441104
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGG20180.4574884834415467No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGG12380.28065943632340673No Hit
GTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGTCGCGTGCCGGCCGGGGGACGGACTGGGAACG11120.2520947440966303No Hit
ACCTATACCCGGCCGTCGGGGCAAGCGCCAGGCCCCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCCGGG11060.25073452065725993No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA9620.2180891581123726No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTC9400.2131016721680148No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGAGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTT9050.20516703543835466No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCAGTCCGTCCCCCGGCCGGCACG8680.19677899089557113No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCAGTCCG8220.18635061119373209No Hit
TCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGTCGCGTGCCGGCCGGGGGACGGACTGGGAACGG7500.1700279299212884No Hit
TCCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGG7460.16912111429504154No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGACGACCCGCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCACCGACAG7370.16708077913598607No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT7330.1661739635097392No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCCGCCCACCCT7120.1614131814719431No Hit
GTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG6810.15438536036852987No Hit
GTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGTCGCGTGCCGGCCG6680.15143820958322754No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA6630.15030469005041897No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGG6450.14622401973230803No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCC6370.1444103884798143No Hit
CCCTGGAAACGACTTAGTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGC6340.14373027676012912No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCT6030.13670245565671588No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCAGTCCGTCCCCC5900.13375530487141354No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAA5820.1319416736189198No Hit
AAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC5810.13171496971235808No Hit
TACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGGCCCCCGAGGGGGCCGTTCCCAGTCCGTCCCCCGGCCG5720.12967463455330264No Hit
AGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGC5660.12831441111393233No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAA5590.1267274837680003No Hit
ATCCTAAGAGACGGGGGAAGCCCGTCCGACAGCGCGTTCGCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC5240.11879284703834017No Hit
GAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACGC5110.11584569625303782No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCCGGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTG4880.11063150640211832No Hit
GCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT4870.1104048024955566No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGA4800.10881787514962458No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCGATGCCTCTAATCATTGGCTTTACCCG4740.10745765171025427No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGCGCGA4710.10677753999056912No Hit
GTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCCGCCCACCCTGGAAACGACT4560.10337698139214334No Hit
GTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCCCACGCCCGGTCGTACTCATAAC4450.10088323841996447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)