FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002129102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435371
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86851.9948503689956383No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC24330.5588337303127677No Hit
CCCCAGGGACCGTCTGCAGCCTCCTGCTCCTCGGCATGCTCTGGCTGGAC16620.38174338667481295No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT13870.31857886721899253No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT10900.25036118620670644No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC10320.23703921483056978No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA9920.22785164836426863No Hit
ATGGGACCTCCGCCAGGAACTGCAGGCCCACCTGTCTGCAACCCAGCTGA9860.22647351339432345No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC8840.2030452189052555No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA8270.18995293669077637No Hit
TCCCAGAGGATGTCCTGAAGAAACTTCCCCAGGGCCTGGCTGTGCTGCTG8270.18995293669077637No Hit
AACAGTCGTGGGAGTTGCTGCAGAAGCAAGCGAAAAGCCAGATGAGCGCT7620.175023141183037No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG7330.16836215549496866No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7300.16767308800999609No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA6820.1566480082504347No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC6440.14791982010744859No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT6400.14700106346081848No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT6300.1447041718442432No Hit
CTTCTAAACTTAGAGAGAGGTGAGTAAGGCTTGTGGGCGATCACTTGTCG6150.14125883441938025No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG6010.13804318615617484No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC5990.13758380783285978No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5720.1313822004681065No Hit
GTCGTGGGAGTTGCTGCAGAAGCAAGCGAAAAGCCAGATGAGCGCTTCTA5710.13115251130644898No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC5570.1279368630432436No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG5220.11989774238523006No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG5110.11737116160699725No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC5020.11530395915207949No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA5000.11484458082876443No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT4980.11438520250544937No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4950.11369613502047679No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4920.1130070675355042No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4860.11162893256555903No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA4790.11002110843395634No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG4540.1042788793925181No Hit
TCCTGAGCTTGTACAACAGTCGTGGGAGTTGCTGCAGAAGCAAGCGAAAA4450.10221167693760035No Hit
ACACCAGAGAGTCCAGCAGAGAAAGGAGTCGAAGAAGCCACCAGCCAAGC4410.10129292029097024No Hit
GAGTAAGGCTTGTGGGCGATCACTTGTCGGCTGGGGCCTCTTTGGCCTCT4410.10129292029097024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)