FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002129103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435371
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51541.1838179391829038No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC22640.5200162619926454No Hit
CCCCAGGGACCGTCTGCAGCCTCCTGCTCCTCGGCATGCTCTGGCTGGAC13430.30847254410606123No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT13320.3059459633278284No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC9670.22210941932283043No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT9650.22165004099951535No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA9330.21429998782647444No Hit
ATGGGACCTCCGCCAGGAACTGCAGGCCCACCTGTCTGCAACCCAGCTGA8080.18558884261928332No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC7470.17157780375817408No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA7330.16836215549496866No Hit
AACAGTCGTGGGAGTTGCTGCAGAAGCAAGCGAAAAGCCAGATGAGCGCT6950.15963396735198257No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG6810.15641831908877715No Hit
TCCCAGAGGATGTCCTGAAGAAACTTCCCCAGGGCCTGGCTGTGCTGCTG6330.14539323932921577No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC6110.14034007777275012No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT5930.1362056728629146No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5930.1362056728629146No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT5880.13505722705462697No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG5690.13069313298313393No Hit
GTCGTGGGAGTTGCTGCAGAAGCAAGCGAAAAGCCAGATGAGCGCTTCTA5690.13069313298313393No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA5680.1304634438214764No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC5650.12977437633650382No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5440.12495090394169571No Hit
CTTCTAAACTTAGAGAGAGGTGAGTAAGGCTTGTGGGCGATCACTTGTCG5310.12196494484014782No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5120.11760085076865479No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA4860.11162893256555903No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4740.10887266262566868No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4730.10864297346401117No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG4710.1081835951406961No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA4500.10336012274588799No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.10267105526091541No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC4420.10152260945262775No Hit
GAGTAAGGCTTGTGGGCGATCACTTGTCGGCTGGGGCCTCTTTGGCCTCT4420.10152260945262775No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT4380.10060385280599765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)