FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002129143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320147
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55641.7379516284706713No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8430.263316538964913No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG7650.23895273108915593No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5110.1596141772373316No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT3830.1196325438001918No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA3790.11838311775528117No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG3780.11807076124405351No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT3730.11650897868791524No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC3560.11119891799704512No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC3550.11088656148581745No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA3360.104951787772492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)