FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002129217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311414
Sequences flagged as poor quality0
Sequence length75
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG30190.9694490292665069Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG26450.8493516669128556Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.5735130726300038No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14170.45502128998696273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11660.374421188514325Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11000.35322753633426884Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10530.3381350870545319Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.31116134791627864No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG9120.2928577392153211Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG8940.28707765225712395Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC8900.2857931884886357Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT8690.27904975370407237Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT8510.2732696667458753Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG7410.23794691311244837Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.23698356528608222No Hit
GTCATATTGAGAGAAGCTATTGGACGAAGCGGGAGCAGCAGTAAAAGCAGCGCAAAACGCCTAACCCAATAGCTT7060.2267078551381762No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6410.20583531890024212Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5650.18143050729896537Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCACATACGGTCATATTGAGAGAAGCTATTGGGTTAGGCGTTTTGCGCTGCTTT5560.1785404638198668No Hit
GTCATATTGAGAGAAGCTATTGGGTTAGGCGTTTTGCGCTGCTTTTACTGCTGCTCCCGCTTCGTCCAATAGCTT5440.17468707251440205No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5340.17147591309318141Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5120.16441136236649603Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCACATACGGTCATATTGAGAGAAGCTATTGGGTTAGGCGTTTTGCGCTGCTTTTACTGCTGCTCCC4550.14610775366553846No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC4090.1313364203279236Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT4050.13005195655943536Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3870.12427186960123822Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGCACATACGGTCATATTGAGAGAAGCTATTGGGTTAGGCGTTTTGCGCTGCTTTT3770.1210607101800176No Hit
ACGCAGAGTACATGGGCACATACGGTCATATTGAGAGAAGCTATTGGGTTAGGCGTTTTGCGCTGCTTTTACTGC3730.11977624641152936No Hit
CTATTGGACGAAGCGGGAGCAGCAGTAAAAGCAGCGCAAAACGCCTAACCCAATAGCTTCTCTCAATATGACCGT3380.10853718843725715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)